Browsing by Author "Soulimane, T."
Now showing items 1-9 of 9
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Detection of a photostable five-coordinate heme a3-Fe-Co species and functional implications of His384/α10 in CO-bound ba 3-cytochrome c oxidase from Thermus thermophilus
Ohta, T.; Pinakoulaki, Eftychia; Soulimane, T.; Kitagawa, T.; Varotsis, Constantinos (2004)Resonance Raman (RR) spectra are reported for the fully reduced carbon monoxy derivative of ba3-cytochrome c oxidase from Thermus thermophilus. The RR spectra show the formation of a photolabile six-coordinate heme-CO and ...
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Detection of the His-heme Fe2+-NO species in the reduction of NO to N2O by ba3-oxidase from thermus thermophilus
Pinakoulaki, Eftychia; Ohta, T.; Soulimane, T.; Kitagawa, T.; Varotsis, Constantinos (2005)Reaction pathways in the enzymatic formation and cleavage of the N-N and N-O bonds, respectively, are difficult to verify without the structure of the intermediates, but we now have such information on the heme a3 2+-NO ...
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Evidence for the presence of two conformations of the heme a 3-CuB pocket of cytochrome caa 3 from Thermus thermophilus
Pavlou, A.; Soulimane, T.; Pinakoulaki, Eftychia (2011)Resonance Raman (RR) and "light" minus "dark" Fourier transform infrared (FTIR) difference spectra are reported for the CO-bound caa3 oxidase from Thermus thermophilus. Two Fe-CO stretching modes at 518 and 507 cm-1, the ...
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Article
Fourier transform infrared (FTIR) and step-scan time-resolved FTIR spectroscopies reveal a unique active site in cytochrome caa3 oxidase from Thermus thermophilus
Pinakoulaki, Eftychia; Soulimane, T.; Varotsis, Constantinos (2002)Fourier transform infrared (FTIR) and step-scan time-resolved FTIR difference spectra are reported for the [carbonmonoxy]cytochrome caa3 from Thermus thermophilus. A major C-O mode of heme a3 at 1958 cm-1 and two minor ...
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Observation of ligand transfer in ba 3 oxidase from thermus thermophilus: Simultaneous FTIR detection of photolabile heme a 3 2+-CN and transient CuB 2+-CN complexes
Loullis, Andreas; Noor, M. R.; Soulimane, T.; Pinakoulaki, Eftychia (2012)FTIR and light-minus-dark FTIR spectroscopy have been employed to investigate the reaction of oxidized and fully reduced ba3 oxidase with cyanide. The characterization of the structures of the bound CN- in the binuclear ...
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Observation of the equilibrium CuB-CO complex and functional implications of the transient heme a3 propionates in cytochrome ba3-CO from Thermus thermophilus. Fourier transform infrared (FTIR) and time-resolved step-scan FTIR studies
Koutsoupakis, Constantinos; Stavrakis, Stavros; Pinakoulaki, Eftychia; Soulimane, T.; Varotsis, Constantinos (2002)We report the first evidence for the existence of the equilibrium CuB 1+-CO species of CO-bound reduced cytochrome ba3 from Thermus thermophilus at room temperature. The frequency of the C-O stretching mode of CuB 1+-CO ...
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Simultaneous resonance Raman detection of the heme a3-Fe-CO and CuB-CO species in CO-bound ba3-cytochrome c oxidase from Thermus thermophilus: Evidence for a charge transfer CuB-CO transition
Pinakoulaki, Eftychia; Ohta, T.; Soulimane, T.; Kitagawa, T.; Varotsis, Constantinos (2004)Understanding of the chemical nature of the dioxygen and nitric oxide moiety of ba3-cytochrome c oxidase from Thermus thermophilus is crucial for elucidation of its physiological function. In the present work, direct ...
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The structure of a ferrous heme-nitro species in the binuclear heme a3/CuB center of ba3-cytochrome c oxidase as determined by resonance Raman spectroscopy
Loullis, Andreas; Noor, M. R.; Soulimane, T.; Pinakoulaki, Eftychia (2015)Members of the cytochrome c oxidase family exhibit nitrite reductase activity. In this work, we have characterized a ferrous heme a3-nitro species in ba3-oxidase by resonance Raman spectroscopy. This provides the first ...
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The structure of the hyponitrite species in a heme Fe-Cu binuclear center
Varotsis, Constantinos; Ohta, T.; Kitagawa, T.; Soulimane, T.; Pinakoulaki, Eftychia (2007)NO laughing matter: The enzymatic production of laughing gas (N 2O) from two molecules of NO requires the formation of the N-N bond and the subsequent cleavage of the N-OH bond. Resonance Raman spectroscopic studies have ...